Hemadsorption of mumps virus examined by light and electron microscopy.

Electron Microscopic Study of Measles Virus Infection: Cell Fusion and Hemadsorption

Virus-induced cell fusion has been studied after infection of Vero cells with measles virus. Scanning and transmission electron microscopy were combined with immunoperoxidase labeling of measles antigens to correlate viral production and distribution of virus-induced erythrocyte binding sites with progress of fusion-Release of infectious virus started before syncytia were detected and decreased...

Hemadsorption immunosorbent technique for determination of mumps immunoglobulin M antibody.

We used hemadsorption immunosorbent technique (HIT) to detect mumps immunoglobulin M (IgM) antibody. IgM from human sera was adsorbed into anti-human IgM-coated wells in plates, and mumps-specific IgM was detected by adding mumps virus hemagglutinin and guinea pig erythrocytes consecutively. Specific IgM-positive sera showed hemadsorption, whereas negative sera showed hemagglutination. All 81 p...

Electron microscopy of African swine fever virus hemadsorption.

A hemadsorption reaction for African swine fever virus (ASFV) was developed by W. A. Malmquist and D. Hay (Am. J. Vet. Res. 21: 104, 1960), and was later modified by W. R. Hess and D. E. DeTray (Bull. Epizootic Diseases Africa 8:317, 1960), as a diagnostic test for African swine fever. Specific hemadsorption takes place with ASFV-infected swine bone marrow and leukocyte (buffy coat) cell cultur...

Comparison of Micro Neutralization Test with Hemadsorption-Inhibition Test, and Immunoflrorescence Technique for Detecting Mumps Antibodies

New tests for characterization of mumps virus antibodies: hemolysis inhibition, single radial immunodiffusion with immobilized virions, and mixed hemadsorption.

Hemolysis inhibition (HLI), single radial immunodiffusion (SRID) with immobilized virions, and mixed hemadsorption tests were used for measuring antibodies against mumps virus. Rabbit hyperimmune sera against mumps and early and late human convalescent sera were analyzed. All three tests identified antibodies against both hemagglutinin and the second major envelope component, hemolysin (fusion ...